松毛虫赤眼蜂-Wolbachia共生菌互作体系对赤眼蜂生物学特性和Wolbachia定植的影响

he parasitic eggs, emergence rate and longevity of Matsumura were compared with the Wolbachia-infected and the non-infected lines under the temperatures of 20, 25 and 30℃. Thelytokous line had been bred for about 20, 80and 300 generations in the laboratory. The wsp gene of Wolbachia was used to verify the genetic stability of thelytokous line of T. dendrolimi. The results showed that the higher generation, the more parasitic eggs of thelytokous line of T. dendrolimi. The gap of parasitic eggs between Wolbachia-infected and non-infected lines of T. dendrolimi became smaller than before. At 25℃, for 300 generations, the number of parasitic eggs of thelytokous line of T. dendrolimi was the highest(47.78 grains). The emergence rate of Wolbachia-infected and non-infected lines T. dendrolimi was significantly different in the same conditions. But the emergence rate was not significantly different for different temperatures and generations. The emergence rate of thelytokous line of T. dendrolimi was the highest as 20 generations at 20℃ (89.62%), the lowest as 80 generations at 30℃ (73.39%).The longevity of thelytokous line of T. dendrolimi was significantly increased at 20℃ and 30℃.High temperature reduced the longevity of T. dendrolimi. Furthermore, the longevity of thelytokous line of T. dendrolimi was the longest as 300 generations at 20℃ (1.49d), the shortest as 20enerations at 30℃ (0.60d).