旋毛虫及其虫源性蛋白对盲肠结扎穿孔诱导的小鼠脓毒症的影响
目的 观察旋毛虫及其虫源性蛋白对盲肠结扎穿孔（CLP）诱导的小鼠脓毒症的影响。 方法 80只雄性BALB/c小鼠随机分为假手术组、CLP组、旋毛虫肌幼虫（ML）预感染组蛋白（SMP）处理组和排泄分泌蛋白（MES）处理组。 ML预感染组于术前28 d经口感染300条旋毛虫肌幼虫，其余各组分别于术后30 min腹腔注射PBS或SMP（25 μg/只）或MES（25 μg/只）。观察小鼠术后状态和 72 h 生存率，检测术后 12 h 小鼠血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、尿素氮（BUN）和肌酐（Cr）水平及TNF-α、IL-6、IL-1β、IL-10、TGF-β水平，观察小鼠肝和肾组织病变。 结果 与假手术组相比，CLP组72 h生存率降低，血清中ALT、AST\BUN和Cr水平及细胞因子TNF-α、IL-6、IL-1β、IL-10和TGF-β水平均明显升高（P<0.05）。肝中肝索排列紊乱，肝细胞水肿，肾中部分血管球皱缩，肾小管细胞水肿。与CLP组相比，ML预感染组血清中ALT、AST、Cr、TNF-α和 IL-1β水平降低，IL-10 和 TGF-β水平升高（P<0.05）；SMP 处理组血清中 ALT、AST、Cr、TNF-α和 IL-1β水平降低，TGF-β水平升高（P<0.05）；MES处理组72 h生存率明显升高，血清中ALT、AST、BUN、Cr、TNF-α、IL-6和IL-1β水平明显降低，IL-10和TGF-β水平明显升高（P<0.05），肝和肾组织结构损伤明显减轻。 结论 旋毛虫及其虫源性蛋白可减少CLP诱导的脓毒症小鼠血清中促炎因子的释放，促进免疫调节因子的释放，其中MES效果更为显著，并能减轻肝和肾结构和功能的损伤。
Objective To observe the effect of Trichinella spiralis and its worm-derived proteins on cecal ligation and puncture(CLP)-induced sepsis in mice. Methods Eighty male BALB/c mice were randomly divided into sham-operated group, CLP group, Trichinella spiralis muscle larvae (ML) pre-infection group (ML + CLP group), soluble muscle larvae proteins (SMP) treatment group (SMP + CLP group) and excretory-secretory proteins (MES) treatment group (MES + CLP group). In ML + CLP group, the mice were orally infected with 300 Trichinella spiralis muscle larvae at 28 days before CLP and those in the other groups were intraperitioneally injected with PBS or SMP (25 g/mice) or MES (25 g/mice) 30 min after CLP. The general condition and 72-h survival after CLP of the mice were observed. The levels of alanine transaminase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN), creatinine (Cr), TNF-, IL-6, IL-1, IL-10 and TGF- were measured at 12 h after the operation, and the pathological changes of the liver and idney were observed. Results Compared with the sham-operated mice, the mice in CLP group showed decreased 72-h survival, obviously increased ALT, AST, BUN, Cr, TNF-, IL-6, IL-1, IL-10, and TGF- with hepatic cords disorder, hepatocytes swelling, glomerulus shrinkage, and renal tubular cell edema. Compared with CLP group, the mice in ML+CLP group showed lowered levels of ALT, AST, Cr, TNF- and IL-1 and increased levels of IL-10 and TGF-; in SMP + CLP group, the levels of ALT, AST, Cr, TNF- and IL-1 were decreased and TGF- increased. In MES + CLP group, the mice showed obviously increased 72-h survival with lowered levels of ALT, AST, BUN, Cr, TNF-, IL-6 and IL-1, increased levels of IL-10 and TGF-, and alleviated liver and kidney damages. Conclusion Trichinella spiralis and its worm-derived proteins can decrease the levels of pro-inflammatory cytokines and increase immunomodulatory cytokines, and MES has more potent effect to reduce structural and functional damages of the liver and kidney.
张慧、宋迪、方强、褚亮、王黎源、吴琦、杨小迪、李楠、仇大鹏、贺文欣、李徽徽、万勇坤
基础医学微生物学
脓毒症旋毛虫盲肠结扎穿孔排泄分泌蛋白促炎因子免疫调节因子
张慧,宋迪,方强,褚亮,王黎源,吴琦,杨小迪,李楠,仇大鹏,贺文欣,李徽徽,万勇坤.旋毛虫及其虫源性蛋白对盲肠结扎穿孔诱导的小鼠脓毒症的影响[EB/OL].(2018-01-25)[2025-08-16].https://chinaxiv.org/abs/201801.00696.点此复制
评论