Distinct gut metagenomics and metaproteomics signatures in prediabetics and treatment-na?ve type 2 diabetics
Distinct gut metagenomics and metaproteomics signatures in prediabetics and treatment-na?ve type 2 diabetics
Abstract BackgroundThe gut microbiota plays important roles in modulating host metabolism. Previous studies have demonstrated differences in the gut microbiome of T2D and prediabetic individuals compared to healthy individuals, with distinct disease-related microbial profiles being reported in groups of different age and ethnicity. However, confounding factors such as anti-diabetic medication hamper identification of the gut microbial changes in disease development. MethodWe used a combination of in-depth metagenomics and metaproteomics analyses of faecal samples from treatment-na?ve type 2 diabetic (TN-T2D, n=77), pre-diabetic (Pre-DM, n=80), and normal glucose tolerant (NGT, n=97) individuals to investigate compositional and functional changes of the gut microbiota and the faecal content of microbial and host proteins in Pre-DM and treatment-na?ve T2D individuals to elucidate possible host-microbial interplays characterising different disease stages. FindingsWe observed distinct differences characterizing the gut microbiota of these three groups and validated several key features in an independent TN-T2D cohort. We also demonstrated that the content of several human antimicrobial peptides and pancreatic enzymes differed in faecal samples between three groups, such as reduced faecal level of antimicrobial peptides and pancreatic enzymes in TN-T2D. InterpretationOur findings suggest a complex, disease stage-dependent interplay between the gut microbiota and the host and emphasize the value of metaproteomics to gain further insight into interplays between the gut microbiota and the host. FundingNational Key Research and Development Program of China, No. 2017YFC0909703, Shenzhen Municipal Government of China, No. JCYJ20170817145809215, and National Natural Science Foundation of China, No. 31601073.
Fang Chao、Yang Ziyi、Hou Yong、Zhong Huanzi、Lu Yan、Zhao Yue、Zou Hua、Hu Yihe、Zhang Jun、Wang Jian、Liu Siqi、Jia Huijue、Liu Fang、Chen Bing、Wu Jiegen、Zhou Baojin、Shi Zhun、Li Junhua、Madsen Lise、Ren Huahui、Hou Guixue、Xu Xun、Bao Xiao、Zi Jin、Gao Yan、Brix Susanne、Yang Huanming、Yang Fangming、Kristiansen Karsten、Chen Jiayu
BGI-Shenzhen||China National GeneBank||Laboratory of Genomics and Molecular Biomedicine, Department of Biology, University of CopenhagenBGI-Shenzhen||China National GeneBankBGI-Shenzhen||China National GeneBankBGI-Shenzhen||China National GeneBank||Laboratory of Genomics and Molecular Biomedicine, Department of Biology, University of CopenhagenSuzhou Centre for Disease Control and PreventionBGI-Shenzhen||China National GeneBankBGI-Shenzhen||BGI Education Centre, University of Chinese Academy of SciencesSuzhou Centre for Disease Control and PreventionSuzhou Centre for Disease Control and PreventionBGI-Shenzhen||James D. Watson Institute of Genome SciencesBGI-Shenzhen||China National GeneBankBGI-Shenzhen||China National GeneBankSuzhou Centre for Disease Control and PreventionBGI-Shenzhen||China National GeneBankBGI-ShenzhenBGI-Shenzhen||China National GeneBankBGI-Shenzhen||China National GeneBankBGI-Shenzhen||China National GeneBank||School of Biology and Biological Engineering, South China University of TechnologyBGI-Shenzhen||Laboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen||Institute of Marine ResearchBGI-Shenzhen||China National GeneBank||Laboratory of Genomics and Molecular Biomedicine, Department of Biology, University of CopenhagenBGI-Shenzhen||China National GeneBankBGI-Shenzhen||China National GeneBankChina National GeneBankBGI-Shenzhen||China National GeneBankSuzhou Centre for Disease Control and PreventionDepartment of Biotechnology and Biomedicine, Technical University of DenmarkBGI-Shenzhen||James D. Watson Institute of Genome SciencesBGI-Shenzhen||BGI Education Centre, University of Chinese Academy of SciencesBGI-Shenzhen||China National GeneBank||Laboratory of Genomics and Molecular Biomedicine, Department of Biology, University of CopenhagenChina National GeneBank
医学研究方法基础医学微生物学
Metagenomicsmetaproteomicsprediabetestreatment-na?ve type 2 diabetes
Fang Chao,Yang Ziyi,Hou Yong,Zhong Huanzi,Lu Yan,Zhao Yue,Zou Hua,Hu Yihe,Zhang Jun,Wang Jian,Liu Siqi,Jia Huijue,Liu Fang,Chen Bing,Wu Jiegen,Zhou Baojin,Shi Zhun,Li Junhua,Madsen Lise,Ren Huahui,Hou Guixue,Xu Xun,Bao Xiao,Zi Jin,Gao Yan,Brix Susanne,Yang Huanming,Yang Fangming,Kristiansen Karsten,Chen Jiayu.Distinct gut metagenomics and metaproteomics signatures in prediabetics and treatment-na?ve type 2 diabetics[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/666263.点此复制
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