纳米硒对性成熟前波尔山羊公羔生殖机能发育的影响

{Objective} An experiment was conducted to study the effect of nano-selenium on reproductive function in Bora Goat ram from weaned to sexual maturity. {Method}Total 20 healthy goats were randomly divided into two treatments in 10 replications, and fed the basal diet supplementation with 0 (control) or 0.3 ppm Se from nano-selenium. The experiment lasted 90 d before 10 weeks pretesting, and the blood samples were collected and seperated serum for sexual hormones every 30 days. The testis obtained, measured the Se concentration and the activity of GSH-Px, fixed and then made the microscopic section, observed the testicular microstructure. At the end of experiment, semen quality in each group were analyzed, and ultrastructure of spermatozoa were observed through TEM. {Result}The results showed that the development of seminiferous tubules, interstitial tissue and germ cells were affected by supplement nano-Se in basal diets. Dietary supplementation with nano-Se increased testis Se content, the activity of GSH-Px (P<0.01) significantly. Testosterone,luteinizing hormone and follitropin were not affected by supplementation Se during the phase of 30-60 d, and increased significantly in the phase of 90 d compared with control. Sperm activity increased 10% and abnormalty sperm decreased 27% respectively(P<0.05), and sperm quantity and density didn’t change. The longitudinal sections of midpiece of ram sperm mitochondria showed the change of the biological characters and arrange loosely. {Conclusion}These results indicated that supplementation nano-Se affected the reproductive function of prepubertital ram, and improved the sperm quanlity. The mechanism needs further research.