基于rGO/Hemin/AuNPs复合材料的电化学传感器用于检测DNA

Using a one-pot strategy synthesized ternary reduced graphene oxide-Hemin-Au (rGO-H-Au) nanohybrids by simply heating the mixed solution of GO, hemin and HAuCl4 under alkaline conditions, without adding any additional surfactant, stabilizing agent and reducing agents. In this composite, GO was reduced to rGO. rGO act as a supporting material, which overcame the disadvantages of Hemin insoluble in water and inhibited the aggregation of AuNPs. Under normal conditions, the composite has the effect of catalyzing H2O2 decomposition, while the bases in single-stranded DNA can interact with rGO through π-π stacking and inhibit the activity of the composite to catalyze H2O2 decomposition.While in the presence of targets, the single-stranded DNA combineed with the targets away from the surface of the rGO-H-AuNPs composite, and the catalytic activity of rGO-H-AuNPs composite recoved. When specific DNA strands are detected, the DNA strand that binds to the rGO-H-AuNPs composite could be replaced with its complementary strand. Based on this, a novel electrochemical sensor based on rGO-H-AuNPs composite was constructed for detecting DNA.