南极大磷虾胰蛋白酶促进皮肤创伤愈合作用研究

Objective To investigate the effects of the Antarctic krill trypsins on cutaneous wound healing. Methods The animal model of full-thickness skin defect was established with mechanical trauma. Each rabbit was cut four wounds which were treated with crude tryspins, pure trypsins, papin and saline. Then pathological morphology change of the wounds were observed, the scars were rated with the Vancouver Scar Scale (VSS), the wounds area was measured, the histological change were observed with tissue sections, and the EGF and bFGF contents of wound skin tissue were essayed. Results At the fiftieth and twentieth day after modeling, the effects of the crude and pure trypsins on reducing epithelite and enhancing healing rate, were better than those of the papain and saline. At the tenth and fiftieth day after modeling, the crude and pure trypsins promoted the expression of EGF and b FGF. Conclusion The results indicated that the crude and pure trypsins from the Antarctic krill can significantly promote the cutaceous wound healing, the effects of the crude trypsins were the best, which can reduce the healing time.