延边黄牛阉牛、公牛背最长肌差异表达基因的筛选与鉴定
Screening and identification of the differentially expressed genes in Longissimus dorsi of Yanbian steers and bulls
为筛选延边黄牛阉牛、公牛背最长肌组织差异表达基因，从基因表达水平揭示阉牛和公牛不同性状差异的遗传基础。文章应用引物退火控制技术(ACP)分别检测了3头30月龄延边黄牛阉牛、公牛背最长肌组织的mRNA表达水平变化。利用20对随机引物差异显示扩增下，共获得10条ESTs，其中8个为已知的ESTs。对NADH脱氢酶亚单位6(ND6)、β-肌球蛋白重链(MYH7)、NDUFS8(NADH dehydrogenase Fe-S protein 2)和α-肌动蛋白1(ACTA1)进行了荧光定量PCR检测，结果显示ACTA1和NDUS8 在阉牛组背最长肌中的表达量显著高于公牛组（P＜0.05）；ND6和MYH7 在公牛组背最长肌中的表达量显著高于阉牛组（P＜0.05）。结果提示，这些基因参与延边黄牛阉割前后肉质性状差异的重要调控基因。
his experiment was conducted study was to screen longissimus muscle differentially expressed genes of Yanbian steers and bulls, and reveal its genetic basis of different traits in gene expression levels. Annealing control primer (ACP) system was applied to screen differentially expressed genes of longissimus muscle of 30 months old Yanbian cattle. Ten DEGs were Using 20 random primers. In these genes, eight were already known,. The expressions of the NADH dehydrogenase subunit 6, MYH7, NDUFS8 and ACTA1 genes were observed ssayed by quantitative PCR. The expressions of ACTA1 and NDUFS8 were significantly higher in the steers (P＜0.05), The expressions of the NADH dehydrogenase subunit 6 and MYH7 genes were significantly higher in the bulls (P＜0.05). All the ESTs were screened by ACP system were considered as the most important genes involved in meat quality differences between steers and bulls Yanbian cattle.
田万年、严昌国、李香子
畜牧、动物医学遗传学生物科学研究方法、生物科学研究技术
延边黄牛引物复性控制技术差异表达基因荧光定量PCR
Yanbian yellow cattleannealing control primer (ACP) systemdifferential expression genes(DEGs)Real-time quantitative PCR
田万年,严昌国,李香子.延边黄牛阉牛、公牛背最长肌差异表达基因的筛选与鉴定[EB/OL].(2014-02-01)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201402-20.点此复制
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