巴戟甲素对AD模型的保护作用及机制探讨

Objective：This study aims to investigate protective effect of Bajijiasu on Aβ42-induced SH-SY5Y cell damage and the underlying mechanism. Methods: SH-SY5Y cells were cultured with Bajijiasu for 12h, and LRP1and APP expression was determined by Western Blot, and changes in real-time PCR detection of SH-SY5Y cells LRP1 mRNA expression. Aβ42 was added into SH-SY5Y cells culture solution followed by Bajijiasu. Aβ42 in intracellular levels of extracellular was determined by ELISA. And it was observed the translation of Aβ42 after entering cells and co-localization with the lysosomal by confocal. Results: Bajijiasu can effectively increase the uptaking and degrading effect of SH-SY5Y cells. Compared with model group Aβ42 conditioned media content,the content of Aβ42 in Bajijiasu group(50, 100μmol o L-1) is relative reduction (P <0.05); compared with the model group content of intracellular Aβ42, the content of Aβ42 in Bajijiasu group (50 μmol o L-1) is relative increase(P <0.05), the Aβ42 intracellular content is not obvious in Bajijiasu group (100μmol o L-1) (P> 0.05). Conclusion: Bajijiasu protect Aβ42-induced SH-SY5Y cells from neurotoxicity injury. The mechanism can effectively increase the LRP1 expression on human neuroblastoma SH-SY5Y cell，which can increase cell endocytosis and lysosomal transport, and reduce Aβ42 deposition and cytotoxic to protecting neurons in the extracellular.