弱离子交换整体柱在线预处理并测定人尿液中头孢唑啉钠

weak ion exchange monolithic column prepared by modifying the GMA-MAA-EDMA (glycidyl methacrylate-methacrylic acid-ethylene glycol dimethacrylate) monoliths with ethylenediamine was applied to remove matrix compounds in biological fluid that otherwise would interfere with the analysis. Water could be used as the washing liquid to flush out matrix compounds while retaining negative charged small molecules. Using this monolithic column, on-line clean-up and screening of cefazolin sodium in human urine samples had been investigated. Chromatography was performed by reversed-phase HPLC on a C18 column with ultraviolet detection at 254nm. The mobile phase was an aqueous solution containing 9.4 mM Na2HPO4 and 5.3 mM citric acid with acetonitrile (87:13 V/V). Calibration curve was linear in the concentration range of 1.0-100µg/mL for cefazolin in human urine The mean recovery was 98.8±3.0% and the LOQ was 0.3µg/mL. Results showed that the weak ion exchange monolithic column could be used for deproteinization and retaining cefazolin sodium in human urine, which provided a simple and fast method for assaying drugs in human urine and plasma.