苦豆碱对膀胱癌EJ细胞增殖与凋亡的影响

Objective To investigate the effects of aloperine on the proliferation and apoptosis of bladder cancer EJ cells and to explore its mechanism. Methods The experimental group was as follows: bladder cancer EJ cells were not treated (group A); 25umol/L aloperine was added (group B); 50umol/L aloperine was added (group C); 100umol/L aloperine was added (group D). The survival rate of the cells was detected by CCK-8. The apoptosis rate was detected by flow cytometry. The expression levels of Bcl-2, Bax, p-Erk1/2 protein were detected by Western-blot. Results CCK-8 results showed that: B, C, D group compared with A group, the cell survival rate were decreased (P < 0.05). The results of flow cytometry showed that the apoptosis rate of B, C and D group was higher than that of A group (P < 0.05). Western-blot results showed that: B, C, D group compared with A group the expression of Bcl-2 and p-Erk1/2 levels were reduced (P < 0.05), the expression of Bax level was increased (P < 0.05). Conclusion Aloperine reduced the survival rate of bladder cancer EJ cells and induced its apoptosis, inhibiting the phosphorylation of Erk1/2 may be the mechanism.