杜仲提取物抗H IV活性成分的分离鉴定
Isola tion and Character iza tion of the An ti2HIV Active
目的:研究杜仲提取有效成分对H IV gp41六股螺旋束的抑制作用,为寻求新的抗H IV病毒进入抑制剂 提供实验依据。方法:将杜仲水提取液分别用石油醚、乙酸乙酯和正丁醇萃取,萃取物干燥称量后制备成浓度为1mg/ml的样品液,用夹心免疫酶联法检测杜仲及其萃取物对H IV gp41六股螺旋束结构形成的抑制作用。结果:发现杜仲抗H IV gp41的活性部分存在于在乙酸乙酯萃取物中,经过醇沉后的上清液失去活性,而沉淀物具有较高的活性。结论:杜仲可抑制H IV gp41六股螺旋束的形成从而起到抗H IV的作用,其活性部位主要在乙酸乙酯萃取物里,活性物质是能被乙醇沉淀的大分子物质。
Objective: To investigate the anti2H IV effects of Eucomm ia ulm oides Oliver, so as to p rovide experimental basis for searching a new efficacious drug for treatment ofA IDS1Methods: Using phytochemistry to isolate compounds from Eucomm ia ulm oides Oliver, the inhibitory activity of Samp les on the H IV gp41 six2helix bundle formation was determined by a modified sandwich EL ISA and PAGE1 Results: The Samp les from Eucomm ia ulm oidesOliver had potent inhibitory activity on the H IV gp41 six2helix bundle for2 mation1 Conclusion: Eucomm ia uloides Oliver can inhibit H IV by targeting H IV gp411.
吕琳、吴曙光
医药卫生理论药学基础医学
HIV gp41病毒进入抑制剂杜仲夹心EL ISA法
H IV gp41H IV entry inhibitor agentsEucomm ia ulm oides OliverSandwich EL ISA
吕琳,吴曙光.杜仲提取物抗H IV活性成分的分离鉴定[EB/OL].(2010-02-04)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201002-221.点此复制
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