利用染色体单片段代换系定位水稻结实率QTL

Seed setting rate (SSR) is one of the key factors determining the commercial potential and is the component of rice yield. However, seed setting rate is susceptible to high temperature, low temperature and drought etc. It is very important to identify the quantitative trait loci (QTL) regulating seed setting rate and study the genetic effect in rice breeding. A set of chromosome segment substitution lines with 9311 as recipient and Nipponbare as donor were genotyped by whole-genome resequencing. By combining the sequencing-based bin-map with a multiple linear regression analysis, we totally detected fourteen QTLs under hydroponic conditions, two for 2010, 5 for 2011 and 7 for 2012, associated with seed setting rate on chromosomes 3, 4, 5, 6, 7, 8, 9, 10 and 11. QTLs explaining the phenotypic effect variance more than twenty percent were qSSR5.1, qSSR7.1, qSSR9.1, qSSR11.1, qSSR3.2, qSSR3.3, qSSR4.1, qSSR8.2 and qSSR9.2. Among these QTLs, qSSR5.1 explained the highest phenotypic effect variance (49.76%) and was mapped in a region of 731482 bp on chromosome 5. The results provided a foundation for fine mapping QTL and subsequent gene cloning.(10 Points, Times New Roman)