孵化期单色绿光刺激通过影响成肌因子和Wnt信号因子表达促进胸肌发育
Monochromatic green light stimuli during incubation accelerates posthatch pectorals muscle growth by altering the expression of myogenic genes and wnt signaling pathway of embryos and neonatal broilers
孵化期单色LED绿光刺激可促进肉仔鸡出雏后的生长发育。本研究选取200枚爱拔益加鸡商品代受精蛋随机分配到黑暗组和LED绿光组（560 nm）2个不同的孵化处理组，以探究孵化期绿光刺激对鸡胚和雏鸡胸肉生长的影响，及成肌调控相关基因和Wnt/β-catenin信号通路关键因子表达的变化规律。光源为LED灯，光照时间为孵化第1天至孵化第19天结束，光照强度为30 lx。结果显示，与黑暗组相比，单色绿光刺激显著提高了出雏早期H3（hatched day 3）和H7天胸肌重和H7天胸肌比率（P<0.05）。绿光组孵化期E15（embryonic day 15）和E17天Pax3基因、E19和H1天Pax7和MyoD基因、H1天MyoG基因和E17至H1天Myf5 等基因的mRNA水平均显著高于对照组（P<0.05）。此外，绿光刺激还可显著提高E15至E19天鸡胚胸肌中Wnt3a和E19天β-catenin基因mRNA水平（P<0.05）。结果表明，孵化期LED绿光刺激可显著促进肉仔鸡出雏早期胸肌的生长发育，其影响作用受Pax3、Pax7、MyoD、MyoG和Myf5等肌形成调控因子和Wnt/β-catenin信号通路关键因子Wnt3a和β-catenin mRNA表达水平升高的调控。
Previous studies have indicated that monochromatic green light-emitting diodes (LED) light stimuli during embryogenesis accelerated post-hatch pectoral muscle growth of broilers. In this study, we further investigated the potential mechanism of this phenomenon by determine the gene expression of paired box genes, myogenic regulatory factors and the key factors of Wnt/β-catenin signaling pathway in the pectoral muscle of embryos and neonatal broilers incubated in dark condition or green LED light stimulation. Arbor Acres fertile broiler eggs (n = 200) were randomly assigned to 2 incubation groups and exposed to continuous green light, or a dark condition (control) from the first day to 19 d of incubation. The light sourced from LED lamps with the intensity of 30 lx at eggshell level. The results showed that green LED light stimuli during embryogenesis increased the total pectorals muscle weight on days H3 and H7, and relative pectorals muscle weight of newly hatched chicks on day H7 (P < 0.05) in comparison to the dark condition. The expression of Pax3 on days E15 (P < 0.05) and E17 (P < 0.01), Pax7 on days E19 and H1 (P < 0.05), MyoD on days E19 and H1(P < 0.05), MyoG on day H1 (P < 0.05), and Myf5 from days E17 to H1 (P < 0.05) was higher in green light group than those in dark condition. In addition, although the no significant difference on Wnt11 mRNA expression was found between treatments, the green LED light stimuli upregulated the mRNA expression of Wnt3a form days E15 to E19, and β-catenin on day E19 (P < 0.05). These results suggest that providing 30 lx green LED light stimuli from the first day to 19 d of incubation may accelerate pectoral muscle growth in embryos and neonatal broilers by enhancing myoblast proliferation and differentiation in the late embryonic stage of embryos and neonatal broilers, which is potentially mediated through the transcriptional upregulation of Pax3, Pax7, MyoD, MyoG and Myf5 and the key factors (Wnt3a and β-catenin) of Wnt/β-catenin signaling pathway..
李蛟龙、张林、高峰、王筱霏、朱旭东
畜牧、动物医学生物科学研究方法、生物科学研究技术分子生物学
鸡胚孵化LED绿光肌肉发育
chick embryo incubation green LED light muscle development
李蛟龙,张林,高峰,王筱霏,朱旭东.孵化期单色绿光刺激通过影响成肌因子和Wnt信号因子表达促进胸肌发育[EB/OL].(2017-02-24)[2025-08-03].http://www.paper.edu.cn/releasepaper/content/201702-159.点此复制
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