Gh14-3-3L30 影响棉花黄萎病抗性

Verticillium wilt affects cotton quality and yield seversly. Verticillium wilt resistance is a complex trait mediated by multiple genes. It is of great significance to deeply explore associated key resistant genes and mechanisms for cotton molecular breeding of disease resistance. Preversly, our lab screened Verticillium wilt induced gene Gh14-3-3L30 by analyzing transcriptome data of cotton roots inoculated with Verticillium dahliae. But the resistance function and mechanism of Gh14-3-3L30 were still unclear. For this, we cloned Gh14-3-3L30 and constructed associated vectors to identify its function about Verticillium wilt resistance. Subcellular localization showed that Gh14-3-3L30 localized in the cytoplasm. The expression of Gh14-3-3L30 was silenced to 60% by virus-induced gene silencing (VIGS) in cotton and then the Verticillium wilt resistance was identifed. Compared with the control group, the disease index of the silenceD group was significantly decreased, and the contents of hydrogen peroxide, POD and lignin were significantly increased. Our results suggested that Gh14-3-3L30 might be involve in the process of Verticillium wilt resistance by regulating the content of ROS and lignin, which provided new genetic resources and theoretical basis for molecular breeding of Verticillium wilt resistance in cotton.