拟南芥中OPR3启动子活性的时空特异性及其诱导

OPR3 has been identified to be the major enzyme in Arabidopsis to catabolize the reduction of 12-oxo-phytodienoic acid ( OPDA), a critical step of jasmonate synthesis. Here we investigated the spatial and temporal expression of OPR3 in Arabidopsis by promoter-GUS fusion. The GUS activity, when driven by OPR3 promoter, was detected in flower organs, roots, and vascular veins of leaves and was highly induced by exogenous MeJA. In addition, the GUS activity was detected in the cells of phloem, indicating that the biosynthesis of jasmonate is mainly in the phloem of Arabidopsis. In consistent with the detection of GUS activity in lateral roots, we found that there are more lateral roots in opr3 than wild type plants. Our findings suggest that the spatial and temporal synthesis of JA is principally determined by OPR3, which will provide a starting point to address the biosynthesis and distribution of JA in plants.