XCR4在急性髓系白血病骨髓间充质干细胞内的表达及分布

Objective To explore the distribution, expression of CXCR4 in bone marrow mesenchymal stem cells (BMSCs) of untreated acute myeloid leukemia (AML) patients and its clinical significance. Methods Bone marrow samples from AML patients with initial diagnosis who were treated before and after chemotherapy, then isolated and cultured BM-MSCs in vitro；Subcellular localization and distribution of CXCR4 on membrane in P2 cells of different groups were observed by using confocal immunofluorescence；Content changes of CXCR4 are detected by using Western blot. Result The distribution of CXCR4 in BM-MSCs of untreated AML group was different from others，did not accumulate in the Golgi apparatus, most of which were located on the cell membrane instead，and protein content was lower than the other two groups. Conclusion The distribution and content change of CXCR4 in BM-MSCs of untreated AML patients may be associated with the change of bone marrow hematopoietic microenvironment，and CXCR4 has a synergistic effect on extramedullary infiltration of leukemic cells.