氟甲烷对厌氧颗粒污泥的甲烷化抑制效应

Methyl fluoride (CH3F), applied at the appropriate concentration, is considered to be a rather specific inhibitor of acetoclastic methanogenesis (AM), while hydrogenotrophic methanogenesis (HM) is unaffected. Although the application of CH3F for quantifying methanogenic pathways in natural anoxic environments has been well established, its effect on methanogenesis in anaerobic granules is few reported. Herein, we tested the inhibition of CH3F on methanogenesis in the mesophilic anaerobic granules and evaluated this inhibitor technique to monitor carbon flow in artificial methanogenic systems. The results showed that the inhibitory effect increased with the initial CH3F concentrations up to 5%. Stable maximum inhibition with 97.5% methanogenic activity reduced was achieved when 5% CH3F was added to the gas, which was higher than that applied in other anoxic systems.