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Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells

Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells

来源:ChemRxiv_logoChemRxiv
英文摘要

Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.

Michael Perkins、Michael Deery、Tara Pukala、Gon?alo Bernardes、Bruno Oliveira、Justin Chalker、Lisa Alcock

Michael PerkinsMichael DeeryTara PukalaGon?alo BernardesBruno OliveiraJustin ChalkerLisa Alcock

10.26434/chemrxiv.7396958.v1

生物化学生物科学研究方法、生物科学研究技术

cysteine sulfenic acidcysteineproteinprotein oxidation

Michael Perkins,Michael Deery,Tara Pukala,Gon?alo Bernardes,Bruno Oliveira,Justin Chalker,Lisa Alcock.Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells[EB/OL].(2018-11-29)[2025-08-02].https://chemrxiv.org/engage/chemrxiv/article-details/60c73f8e9abda2d16af8baf9.点此复制

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