Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells
Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells
Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.
Michael Perkins、Michael Deery、Tara Pukala、Gon?alo Bernardes、Bruno Oliveira、Justin Chalker、Lisa Alcock
Michael PerkinsMichael DeeryTara PukalaGon?alo BernardesBruno OliveiraJustin ChalkerLisa Alcock
生物化学生物科学研究方法、生物科学研究技术
cysteine sulfenic acidcysteineproteinprotein oxidation
Michael Perkins,Michael Deery,Tara Pukala,Gon?alo Bernardes,Bruno Oliveira,Justin Chalker,Lisa Alcock.Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells[EB/OL].(2018-11-29)[2025-08-03].https://chemrxiv.org/engage/chemrxiv/article-details/60c73f8e9abda2d16af8baf9.点此复制
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