梁平柚葡萄糖基转移酶基因的克隆及原核表达
loning and prokaryotic expression of glucose transferase gene from Citrus maxima 'Liang ping'
本文基于GenBank公布的葡萄糖基转移酶（LGT）基因的核苷酸序列，设计并合成特异性引物，以梁平柚成年树幼叶总RNA为模板，通过RT-PCR方法扩增葡萄糖基转移酶基因，将该基因片段克隆到原核表达载体pET-28a(+)上，构建了该基因的融合表达载体pET28a-CmLGT，转化到大肠杆菌BL21(DE3)中。经1.0 mmoloL-1IPTG诱导表达获得大小约为62 kD的目的融合蛋白，为进一步纯化和鉴定目的蛋白及研究其功能奠定了基础。
Based on the nucleotide sequences of glucose transferase gene（LGT）published on GenBank, specific primers were designed and synthesized. The LGT gene was obtained by RT-PCR from total RNA as the template in young leaves of Citrus maxima'Liang ping' adult tree and the fusion expression vector pET28a-CmLGT was constructed by this gene fragment cloned into the prokaryotic expression vector pET-28a(+), which was then transformed into E. coli BL21(DE3).The objective fusion protein with the size of about 62 kD was induced and expressed by 1.0 mmoloL-1 IPTG, laying the foundations for further purification and identification of target protein and studying its function.
马鑫、王斌、眭顺照、李名扬、胡雨晴
生物工程学分子生物学生物化学
葡萄糖基转移酶mLGT基因克隆原核表达
glucose transferaseCmLGT geneCloningProkaryotic expression
马鑫,王斌,眭顺照,李名扬,胡雨晴.梁平柚葡萄糖基转移酶基因的克隆及原核表达[EB/OL].(2011-09-15)[2025-07-21].http://www.paper.edu.cn/releasepaper/content/201109-220.点此复制
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