黄芪甲苷和三七总皂苷中主要有效成分抗PC12细胞氧化损伤的配伍研究

Objective：To explore the effective compatibility and their respective dose of Astragaloside Ⅳ, GinsenosideRb1, GinsenosideRg1 and NotoginsenosideR1 resistance to Oxidative damage by CoCl2 of PC12 cells. Methods：PC12 cells injury were induced by CoCl2.We used Lactate dehydrogenase (LDH) leakage rate as an indicator to study the effective inhibitory concentration of four active ingredients of anti-oxidative damage in PC12 cells and then identify the best compatibility and their dose of four active ingredients according to the uniform design of U8﹡(85). Result：Six different concentrations of the four active ingredients can effectively inhibit the leakage of LDH(p﹤0.05). Furthermore, the inhibition rate of the LDH leakage increases with the increase of doses. The inhibition rate of LDH leakage reach to 80% when the concentration of AstragalosideⅣ and GinsenosideRb1 in 50μmol/L, GinsenosideRg1 and NotoginsenosideR1 in 100μmol/L. The result of multiple regression analysis for the uniform design of U8﹡(85) showed that eight different concentrations of compatibility of four active ingredients can effectively inhibit the leakage of LDH (p﹤0.05).The best effective components dose to against the Oxidative damage of PC12 cells induced by CoCl2 is GinsenosideRg1 in 50μmol/L, Astragaloside Ⅳ in 0.78125μmol/L, GinsenosideRb1 and NotoginsenosideR1 each in 1.5625μmol/L.