水稻类钙调磷酸酶B亚基蛋白基因OsCBL8功能的初步研究

In order to investigate the function of rice OsCLB8 it was used the homozygous T3 transgenic lines with sense OsCLB8 and anti-sense OsCLB8 obtained by Agro-transformation in this study. The results showed that either over-expression or inhibition of OsCBL8 gene in transgenic lines had little effect on main agronomic traits, but significantly decreased the filled grains per panicle and seed set rate in some lines. Semi-quantitative RT-PCR showed that the expression of OsCBL8 extremely increased in sense transgenic seedlings, and decreased to some extent in anti-sense transgenic ones. By evaluation of tolerance to 150 mM NaCl, 20% PEG6000 and low temperature treatments and relevant physiological index in seedlings of transgenic lines, One sense transgenic line, 8F12, with high salt tolerance and one antisense transgenic 8R14 with high drought tolerance were obtained respectively. It was discussed that the OsCBL8 gene may play important role in rice tolerance to abiotic stresses.