白花蛇舌草乙醇提取物对大肠癌耐药裸鼠移植瘤生长的影响
Inhibitory effect of Ethanol Extract of Hedyotis diffusa Willd on colorectal cancer drug-resistant xenograft in nude mice
探讨白花蛇舌草乙醇提取物（EEHDW）对大肠癌耐药裸鼠移植瘤生长的抑制作用及其分子机制。通过构建大肠癌5-氟尿嘧啶（5-FU）耐药细胞皮下移植瘤动物模型，随机分为对照组、EEHDW组、5-FU组、EEHDW联合5-FU组（联合组），通过瘤体体积测量、RT-PCR和IHC等方法计算抑瘤率、检测移植瘤组织中PCNA的表达及CyclinD1、CDK4、p21的mRNA和蛋白表达。与对照组比较，5-FU对移植瘤的生长无明显影响（P>0.05），EEHDW及联合组移植瘤生长显著抑制（P<0.05），且联合组抑瘤效果更显著；EEHDW及联合组明显抑制移植瘤细胞增殖，下调移植瘤组织中Cyclin D1、CDK4、上调p21的mRNA和蛋白表达（P＜0.05）。 EEHDW在体内通过抑制Cyclin D1、CDK4、上调p21表达是其抑制大肠癌耐药移植瘤生长的重要机制。
nude mice drug-resistant subcutaneous xenograft model was established to study the growth inhibitory effect of Ethanol Extract of Hedyotis diffusa Willd (EEHDW). Mice were randomized into 4 groups (Control, EEHDW, 5-FU, and EEHDW-5-FU combination), to calculate the inhibition rate by determination of tumor volume, and to detect PCNA by IHC, and CyclinD1, CDK4 as well as p21 by RT-PCR and IHC. Compared with the control group, 5-FU had few effect on tumor growth (P>0.05), while EEHDW and combination treatment significantly inhibited growth of xenograft (P<0.05), and combination treatment had a better effect; EEHDW and combination treatment significantly inhibited the proliferation of tumor cells, down-regulated cyclinD1, CDK4, and promoted expression of p21 on mRNA and protein levels (P<0.05). The down-regulation of cyclinD1, CDK4 and up-regulation of p21 are important mechanisms by which EEHDW inhibits colorectal cancer drug-resistant xenograft.
林久茂、赖子君、冯健愉、严兆坤、彭军、靳祎祎
肿瘤学药学基础医学
中西医结合白花蛇舌草大肠癌5-氟尿嘧啶肿瘤耐药移植瘤
Integrated MedicineHedyotis diffusa Willdcolorectal cancer5-Fluorouracildrug resistancexenograft
林久茂,赖子君,冯健愉,严兆坤,彭军,靳祎祎.白花蛇舌草乙醇提取物对大肠癌耐药裸鼠移植瘤生长的影响[EB/OL].(2016-07-20)[2025-08-10].http://www.paper.edu.cn/releasepaper/content/201607-215.点此复制
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