根肿菌侵染下菘蓝生物碱合成机制
Mechanism of alkaloid synthesis in Isatis indigotica infected by Plasmodiophora brassicae
为了探究根肿菌胁迫对菘蓝生物碱及其合成关键酶基因表达的影响，该文对根肿菌侵染后0、7、14、21 d 的菘蓝进行病情形态分级、组织学观察、生理生化指标测定以及转录组学和代谢组学分析。结果表明：（1）接菌后0、7、14、21 d 菘蓝根部分别发展为0 级、1 级、3 级、5 级的肿根，且7 d 是皮层入侵的关键时间点。（2）接种根肿菌14 d 后，菘蓝叶内可溶性蛋白、丙二醛含量、超氧化物歧化酶、过氧化物酶、多酚氧化酶和过氧化氢酶活性与同时间对照组比较显著提高，并随着接菌时间的延长呈增加的趋势。（3）代谢组学一共检测到161 种生物碱，其中吲哚类生物碱数量较多；与未接菌相比，菘蓝接菌后7、14、21 d 分别存在16、17、39 种差异代谢物且各组差异代谢物多富集在生物碱和氨基酸代谢通路。（4）转录组测序结果显示：与未接菌相比，菘蓝接菌后7、14、21 d 分别存在2 439、256、6 437 个差异表达基因，这3 组共同富集到11 个生物碱相关的代谢通路；与未接菌相比，接菌后7、14、21 d 有9 个基因（编码4 种酶THS、TAT、YUCCA、ALDH）表达量均上升。以上结果揭示了芸薹根肿菌与菘蓝之间的互作机制，探究了根肿菌对吲哚生物碱合成及其关键酶基因的影响，为后期研究菘蓝根肿病抗性基因及生物碱次生代谢途径奠定基础。
生物化学分子生物学植物学
板蓝根，芸薹根肿菌，抗氧化酶，功能基因，吲哚生物碱，代谢组
张丽琴,赵淑丽,施建莲,李国栋,刘家佳,赵明智.根肿菌侵染下菘蓝生物碱合成机制[EB/OL].(2023-12-26)[2025-10-30].https://chinaxiv.org/abs/202312.00325.点此复制
To explore expression level of alkaloid and its synthetase gene in Isatis indigotica uponPlasmodiophora brassicae Woronin exposure. Our study verified grade of disease severityaccording to morphology. Moreover, histological, physiological, biochemical parameters havebeen collected together with transcriptomics and metabolomic analysis in Isatis indigotica afterinfection at time scale 0, 7, 14, 21 d. Results showed that: (1) After infect with P. brassicae, I.indigotica showed club roots grade in 0, 1, 3, and 5 respectively at 7, 14, 21 d, notably, corticalinvasion occurred on 7 d. (2) Our study revealed when P. brassicae exposes lasting 14 d later, the level of soluble protein and malondialdehyde, along with superoxide dismutase, peroxidase,polyphenol oxidase and catalase activity in I. indigotica increased significantly compared tocontrol group at time depended manner. (3) A total of 161 alkaloids were detected inmetabolomics, among those alkaloids, indoles were noticed as the most abundant form. Therewere 16, 17 and 39 discriminating metabolites have been spotted after infected with P. brassicaeat 7, 14, 21 d, the most discriminating metabolites enriched at alkaloid and amino acid metabolismpathways. (4) Transcriptome analysis showed that there were 2 439, 256 and 6 437 genesexpression alteration at 7, 14, 21 d compared to control, those differentially expressed genesenriched at 11 alkaloids related metabolism pathways. Markedly, expression level of 9 genes(encoding for enzymes thebaine synthase, tyrosine aminotransferase, indole-3-pyruvatemonooxygenase and aldehyde dehydrogenase) were increased after infection at 7, 14, 21 d. Ourresults revealed the interaction between P. brassicae and I. indigotica. The effects of P. brassicaeon indole alkaloids and enzymic genes expression laid ground for studying genes mediate clubroot resistance and alkaloid secondary metabolic pathways in I. indigotica.
Isatidis Radix，Plasmodiophora brassicae，antioxidant enzymes，functional gene，indole alkaloids，metabolome
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