荧光原位杂交技术检测果蝇翅芽基因表达

rosophila larval wing disc is a representative insect small organ to study cell differentiation, cell growth and morphogenesis. RNA in situ hybridization experiment was performed in dissected Drosophila wing discs at 3rd instar using one of the cellular memory genes trithorax. Many factors can influence the in situ hybridization results which include the stage of the materials, probes, time and temperature of prehybridization and hybridization, washing time, and proper controls. trithorax is ubiquitously expressed in the wing disc and negatively regulated by the transcription factor Optomotor-blind. This expression pattern and regulation of trithorax provide a valuable research resource for studying the development of wing disc.