wMel菌株人工转染后在烟粉虱种群中的滴度变化动态

he change in the titre of a foreign Wolbachia strain after transinfecton may be involved in its capability for controlling host population. Herein, the wMel Wolbachia strain from the fruit fly Drosophila melanogaster was artificially transferred into the whitefly Bemisia tabaci. Fluorescence in situ hybridization verified the presence of wMel in the new host; the transinfected whiteflies were then used to establish the isofemale lines. The results based on real-time quantitative PCR showed that the titre of wMel strain reached a low level at G2 and G3 after transinfecton, then rose steadily and reached the peak at G6, but dropped to the minimum at G9 and then slightly recovered after G10. These results suggest that wMel can be transmitted in B. tabaci populations, but its titre fluctuated among different generations, which seems to have affected the transmission of wMel in transinfected offspring populations of B. tabaci.