枳水通道蛋白PIP基因家族成员的克隆及其在硼胁迫下的表达分析

In order to study the relationship of Aquaporins PIP gene family and boron transportation in trifoliate orange [Poncirus trifoliata (L.) Raf.], ten genes of PIP subfamily gene were cloned which includes three PtrPIP1s genes and seven PtrPIP2s genes and analyzed by using Bioinformatics. We found that，PtrPIP1s and PtrPIP2s genes were located on large different branches in phylogenetic tree ,their amino acid sequences were consisted of six alpha-helical transmembrances and two conserved NPA（Asn-Pro-Ala）motifs while PtrPIP1;3,PtrPIP2;5 and PtrPIP2;7 genes were consisted of four alpha-helical transmembrances and one conserved NPA motif.The RT- qPCR analysis of PtrPIPs gene family in different organs under boron stress were studied which were manly in root，stem and leaf (PtrPIP2;7),respectively. All PtrPIP2s genes responed strongly to boron stress whereas PtrPIP1s responed slightly to it. The relative expression levels of PtrPIP1;2 genes were mainly in stem among PtrPIP1s genes. Among PtrPIP2s genes, the highest relative expression of PtrPIP2;2 and PtrPIP2;3gene were in root under boron deficiency and high boron stress,respectively，the PtrPIP2;4 gene was in stem under boron stress which was accordanced with its organ specificity,and the PtrPIP2;7 gene was in leaf under high boron stress which was in stem under boron deficiency. These results show that the members of PtrPIPs genes may have participated in transporting boron acid.?????