包载盐酸阿霉素的明胶-泊洛沙姆脂质体制备与表征

Gelatin-poloxamer nanoliposomes (DOX-nanoliposomes) encapsulated doxorubicin hydrochloride were prepared by novel method, and their characterization were evaluated. Gelatin-poloxamer W/W emulsion combined with twice lyophilization technique were used to prepare DOX-nanoliposomes. Sephadex G-50 column and HPLC were applied to determine the loaded doxorubicin hydrochloride in DOX-nanoliposomes. The characterization of DOX-nanoliposomes was evaluated by the appearance of the DOX-nanoliposome solution, particle size, zeta potential and drug encapsulation efficiency. The results show DOX-nanoliposomes exhibit good elliptical morphology without particle aggregation. The average particle size of DOX-nanoliposomes is (187.02±9.56) nm. Moderately negative zeta potential values (-16.8±1.43) mV are observed. The encapsulation efficiency of DOX-nanoliposomes reached (86.3±2.3)%. W/W emulsion preparation combined with twice freeze-drying technology is suitable for preparing DOX-nanoliposomes with high quality.