应用ITS2序列鉴定垂盆草及其混伪品

Objective: To identify medicinal and edible Sedum sarmentosum and its adulterants, and to secure the clinical efficacy and edible safety of this plant. Methods: The PCR technique was performed to obtain the ITS2 regions and the purified PCR products were sequenced bi-directionally. Sequences were assembled by the CodonCode Aligner and analysed by MEGA5.0. The neighbor-joining phylogenetic tree was constructed based on the Kimura 2-parameter model. Results: The ITS2 regions of S. sarmentosum exhibited obvious difference with those of the adulterants. The intraspecific variation was less than interspecific genetic distance. All samples of S. sarmentosum showed monophyletic on NJ tree, and distinguished from its adulterants. Conclusion: ITS2 can be used as an effective marker for identifying S. sarmentosum and its adulterants, and it has a potential applications in the identification of traditional Chinese medicine.