不同时期添加GTPs对奶牛体外胚胎早期发育的影响

he objective of this study is to examine the effects of green tea polyphenols (GTPs) on in vitro fertilization (IVF) and early embryo development in cow. The different concentrations of GTPs (0, 10, 15, 20 and 25 μM) were added respectively into media during IVF and in vitro culture (IVC) of embryos. The results show that the cleavage rate and blastocyst rate were not significant difference when GTPs are added in IVF media. Addition of 15 μM GTPs to IVC media significantly increased the blastocyst rate, but no further increase in the blastocyst rate when higher concentrations GTPs (20 or 25 μM) are added. Compared with the control group, addition of 15μM GTPs to IVC media significantly increased the GSH content and GR gene expression levels within 7d blastocyst, but supplementation of a higher concentration (25μM) GTPs reduced the GSH content and GR gene expression. These results suggest that addition of appropriate concentration of GTPs can improve the ability of early embryonic development by alleviating oxidative stress during IVC period.