Plap-1/Aspn lineage tracing and single-cell transcriptomics reveals cellular dynamics in the periodontal ligament
Plap-1/Aspn lineage tracing and single-cell transcriptomics reveals cellular dynamics in the periodontal ligament
Summary Periodontal tissue supports teeth in the alveolar bone socket via fibrous attachment of the periodontal ligament (PDL). The PDL contains periodontal fibroblasts and stem/progenitor cells, collectively known as PDL cells (PDLCs), on top of osteoblasts and cementoblasts on the surface of alveolar bone and cementum, respectively. However, the characteristics and lineage hierarchy of each cell type remain poorly defined. This study identified periodontal ligament associated Protein-1 (Plap-1/Aspn) as a PDL-specific extracellular matrix. We generated knock-in mice expressing CreERT2 and GFP specifically in Plap-1-positive PDLCs. Genetic lineage tracing confirmed the long-standing hypothesis that PDLCs differentiated into osteoblasts and cementoblasts. A PDL single-cell atlas defined cementoblasts and osteoblasts as Plap-1-Ibsp+Sparcl1+ and Plap-1-Ibsp+Col11a2+, respectively. Other populations such as Nes+ mural cells, S100B+ Schwann cells, and other non-stromal cells were also identified. RNA velocity analysis suggested that Plap-1highLy6a+ cell population was the source of PDLCs. Lineage tracing of Plap-1+ PDLCs during the periodontal injury model showed periodontal tissue regeneration by PDLCs. Our study defines diverse cell populations in PDL and clarifies the role of PDLCs in periodontal tissue homeostasis and repair.
Tamai Katsuto、Kitayama Tomomi、Miyashita Kazuya、Tomita Kiwako、Ikegami Kentaro、Murayama Masanori A、Iwakura Yoichiro、Yamada Satoru、Olson Lorin E、Murakami Shinya、Takedachi Masahide、Ogawa Shuhei、Bhongsatiern Phan、Sakashita Hiromi、Iwayama Tomoaki、Matsumoto Shuji、Iwashita Mizuho、Shimbo Takashi
Department of Stem Cell Therapy Science, Osaka University Graduate School of MedicineStemRIM Inc.||Department of Stem Cell Therapy Science, Osaka University Graduate School of MedicineStemRIM Inc.Department of Periodontology, Osaka University Graduate School of DentistryStemRIM Inc.Research Institute for Biomedical Sciences, Tokyo University of ScienceResearch Institute for Biomedical Sciences, Tokyo University of ScienceDepartment of Periodontology and Endodontology, Tohoku University Graduate School of DentistryCardiovascular Biology Research Program, Oklahoma Medical Research FoundationDepartment of Periodontology, Osaka University Graduate School of DentistryDepartment of Periodontology, Osaka University Graduate School of DentistryResearch Institute for Biomedical Sciences, Tokyo University of ScienceDepartment of Periodontology, Osaka University Graduate School of DentistryDepartment of Periodontology, Osaka University Graduate School of Dentistry||StemRIM Institute of Regeneration-Inducing Medicine, Osaka UniversityDepartment of Periodontology, Osaka University Graduate School of DentistryDepartment of Periodontology, Osaka University Graduate School of DentistryDepartment of Periodontology, Osaka University Graduate School of DentistryDepartment of Stem Cell Therapy Science, Osaka University Graduate School of Medicine||StemRIM Institute of Regeneration-Inducing Medicine, Osaka University
口腔科学基础医学细胞生物学
periodontal tissuealveolar boneperiodontal ligamentextracellular matrixprogenitor cellstem cellmural cellsingle-cell analysiscell atlaswound healing
Tamai Katsuto,Kitayama Tomomi,Miyashita Kazuya,Tomita Kiwako,Ikegami Kentaro,Murayama Masanori A,Iwakura Yoichiro,Yamada Satoru,Olson Lorin E,Murakami Shinya,Takedachi Masahide,Ogawa Shuhei,Bhongsatiern Phan,Sakashita Hiromi,Iwayama Tomoaki,Matsumoto Shuji,Iwashita Mizuho,Shimbo Takashi.Plap-1/Aspn lineage tracing and single-cell transcriptomics reveals cellular dynamics in the periodontal ligament[EB/OL].(2025-03-28)[2025-05-09].https://www.biorxiv.org/content/10.1101/2022.07.16.500314.点此复制
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