旋毛虫幼虫侵入胃上皮细胞后虫体与细胞蛋白变化的研究*

Objective To observe the invasion and protein changes of larval and cell proteins after invasion of gastric epithelial cells by Trichinella spiralis infective larvae, and to screen the invasion-related proteins. Methods T. spiralis infective larvae were inoculated on monolayer of gastric epithelial cell (SGC-7901), the larval invasion was observed under an inverted microscope after culture at 37 ℃ in 5% CO2 for different time. The larval and cell proteins after culture for 18 h were extracted and analyzed by SDS-PAGE and Western blot. Results When the larvae were cultured for 6 h, the larva's head invaded cell monolayer. The anterior and posterior sheathe of larva was observed after culture for 18 h. The results of SDS-PAGE showed after the larvae were cultured with cells, three additional protein bands were observed, and 3 protein bands was disappeared, compared with larvae cultured only in medium. After the cells were cultured with larvae, three new protein bands were observed, and two protein bands were disappeared. Western blot analysis showed after the larvae were cultured with cells, three additional protein bands (58, 21, 18 kDa) were recognized by sera from infected mice and three protein bands (98, 86, 13 kDa) were not recognized by infection sera compared with proteins from larvae incubated in medium only. After the cells were cultured with larvae, six additional protein bands (96, 62, 40, 34, 29, 22 kDa) were recognized by infection sera and two protein bands (98, 15 kDa) were not recognized by infection sera. Conclusions T. spiralis infective larvae invade the monolayer of gastric epithelial cells cultured in vitro. After the larvae were cultured with cells, additional proteins of larvae and cells may be the invasion-related proteins secreted by the larvae.