拟人参皂苷F11对帕金森病模型大鼠纹状体NADPH氧化酶表达的影响
Effect of Pseudoginsenoside-F11 on striatal NADPH oxidases expression in rat model of Parkinson's Disease
目的：拟人参皂苷F11（Pseudoginsenoside-F11, PF11）是从西洋参茎叶中提取的奥克梯隆型三萜皂苷单体。实验室前期研究发现PF11对帕金森病（Parkinson's disease, PD）具有明显的神经保护作用。NADPH氧化酶的激活是PD脑内活性氧的产生的主要来源，并且是抗氧化剂治疗的作用靶点。本研究考察了PF11对PD模型大鼠纹状体内NADPH氧化酶表达的影响。方法：采用6-OHDA损毁左侧前脑内侧束建立大鼠PD模型，连续三周灌胃给予PF11（3, 6, 12 mg/kg），每日一次。采用western blot方法考察PF11对PD大鼠纹状体NADPH氧化酶蛋白表达的影响来探讨其可能的作用机制。结果：PF11显著抑制PD大鼠纹状体NADPH氧化酶亚基gp91phox和p47phox蛋白表达的上调。结论：PF11改善PD的作用机制可能是通过抑制纹状体内NADPH氧化酶蛋白表达从而抑制氧化应激的发生而实现的。
im: Our previous studies showed that Pseudoginsenoside-F11 (PF11), an ocotillol-type saponin existing in American ginseng, had potent neuroprotective effect on Parkinson's disease (PD). NADPH oxidases are sources of intracellular ROS and potential targets for therapeutic administration of antioxidants. In the present study, the effect of PF11 on 6-OHDA induced alterations in NADPH oxidases expression in striatum of PD rats was investigated. Methods: 6-OHDA was injected into left medial forebrain bundle (MFB) to replicate a rat PD model. PF11 was orally administered at 3, 6, 12 mg/kg once daily for 3 weeks. The NADPH oxidases expression in striatum in PD rats was investigated by using western blot. Results: PF11 inhibited the expression of gp91phox and p47phox in striatum in PD rats. Conclusions: These findings suggested that PF11 might improve PD via its regulating effect on the NADPH oxidases expression in striatum of PD rats and inhibiting the oxidative stress.
王建宇、吴春福、王芳、姚雪春、王昉旸、杨静玉
神经病学、精神病学基础医学药学
药理学拟人参皂苷F11帕金森病NADPH氧化酶
PharmacologyPseudoginsenoside-F11Parkinson's diseaseNADPH oxidases
王建宇,吴春福,王芳,姚雪春,王昉旸,杨静玉.拟人参皂苷F11对帕金森病模型大鼠纹状体NADPH氧化酶表达的影响[EB/OL].(2014-03-04)[2025-08-05].http://www.paper.edu.cn/releasepaper/content/201403-88.点此复制
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