柚皮苷印迹β-环糊精聚合物的制备及性能研究

Rod-like molecularly imprinted polymer (RMIP) was prepared by emulsion polymerization using β-cyclodextrin (β-CD) as a functional polymer, naringin (NG) as a template molecule and hexamethylene diisocyanate (HMDI) as crosslinker. The morphology，pore size distribution and specific surface area of the RMIP were observed by means of scanning electron microscopy (SEM) and BET adsorption apparatus. The interaction between the template molecule and functional polymer was analyzed by the infrared spectrum (IR) and 1H-nuclear magnetic resonance (1H-NMR). Its adsorption property and selectivity were evaluated by equilibrium adsorption experiment method. The obtained results revealed appriapriate affinity and selectivity of RMIP to NG. Scatchard analysis suggests that during RMIP recognition of NG, there were two classes of binding sites. The calculated dissociation constant KD1 and apparent maximum number Bmax1 of binding sites with high affinity were 0.016 mmol/L and 15.31 μmol/g respectively, while KD2 and Bmax2 of binding sites with low affinity were 0.24 mmol/L and 98.41 μmol/g. In 0.02 mg/mL NG solution, the distribution coefficients KD of NG on RMIP and corresponding NIP are 4.38 and 2.86, respectively. The imprinting factor α is 1.53.