静电纺SF/PLCL/Gelma复合纳米纤维膜的构建及性能研究

Objective: To prepare silk fibroin/poly(lactic acid-co- caprolactone)/methacrylic anhydride gelatinnanofiber membrane by electrospinning, and to evaluate its physicochemical and effect of proliferation and osteogenic differentiation on rabbit bone marrow mesenchymal stem cells (rBMSCs). Method: SF, PLCL and Gelma were blended in hexafluoroisopropanol to form G0, G2, G4 and G6 in different mass ratios, andnanofiber membranes were prepared by electrospinning method. The physicochemical properties of the electrospun membranes were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), contact angle, tensile strength, and in vitro degradation. FDA staining assay, CCK-8 assay and AKP activity assay were performed to study the activity, proliferation and osteogenesis of rBMSCs on the fibrous membrane.Results: The SEM results showed that the fibers in each group were smooth and continuous; FTIR and XRD results showed that Gelma was successfully spun into the fiber membrane; the contact angle and degradation tests showed that the groups with Gelma had a larger contact angle and faster degradation than the group without Gelma; Compared with the blank, the G2 group had a significant promoting effect on the proliferation of rBMSCs on the 3th and 5th day; after 7 days of culture, there was no significant difference in the ALP activity of the rBMSCs in each group. Conclusion: The mechanical properties, degradation and hydrophilicity of the fibrous membrane in the G2 group were better, and the biocompatibility was excellent, and it may have a certain promotion effect on the osteogenesis of rBMSCs.