外源水杨酸在灵芝中促进灵芝酸生物合成

We herein demonstrate that salicylic acid (SA) can enhance ganoderic acid (GA) accumulation in Ganoderma lucidum. Following treatment with different concentrations of SA, the GA content was increased 22.72 - 43.04% compared with the control group. When the fungi were treated with 200 μM SA at different times, the GA content was improved 10.21- 35.24% compared with the control group. By choosing the optimum point based on the response surface methodology, the GA content could be increased up to 229.03 μg/100 mg, which was improved 66.38% compared with the control group. When the fungi were treated with 200 μM SA, the transcription levels of key genes in the GA biosynthesis pathway, squalene synthase (sqs), lanosterol (osc) and hydroxy-3-methylglutaryl-Coenzyme A reductase (hmgr), were improved 119.6-, 3.2- and 4.2-fold, respectively. In addition, following treatment with 100 μM SA, the levels of lanosterol and squalene, which are intermediate metabolites of GA biosynthesis, were improved 2.8- and 1.4-fold, respectively. These results indicate that SA can regulate the expression of genes related to GA biosynthesis and increases the metabolic levels of lanosterol and squalene, thereby resulting in the accumulation of GA.