XTH基因家族在妃子笑荔枝花穗不同处理方式的表达分析

Xyloglucan endotransglucosylase/hydrolase (XTH) is an important class of glycoside hydrolase that plays an important role in promoting plant cell wall elongation and responding to stress. In order to study the expression of the XTH family genes in different spike treatments, this study used the Feizixiao litchi as the experimental material. The flower spikes of litchi were treated with sparse flowers and uniconazole treatment respectively. Based on the pre-transcriptome database, 29 LcXTH family members were identified. The expression of LcXTHgene family members in different spikes was studied by real-time PCR (qRT-PCR). And analyzed the response of LcXTH family genes to different spike treatments. The results showed that the expression of LcXTH family members was different in the different developmental stages of the flowering stage after control, sparse treatment and uniconazole treatment, And there are differences in the expression levels of different genes. Among them, members of LcXTH subfamily III, LcXTH subfamily IV (LcXTH2, LcXTH3, LcXTH4, LcXTH5, LcXTH6, LcXTH7, LcXTH9, LcXTH10, LcXTH11, LcXTH12, LcXTH13, LcXTH22 and LcXTH23) and LcXTH20 have higher expression levels, presumably it plays a key role in flower development. LcXTH expression was up-regulated at the 14th day after flowering, We speculatee flowering has a positive regulation effect on LcXTH. Uniconazole treatment inhibited the expression of LcXTH and negatively regulated LcXTH.