胃癌BGC-823药物存活细胞相关特性研究

Objective: to observe the changes of characteristics in BGC-823 drug survival cells treated with short-term doxorubicin, which can provide some new clues for the study of drug resistance in gastric cancer. Methods: The inhibitory effects of different concentrations of doxorubicin on BGC-823 for 48-hour was evaluated by MTT assay，then select IC50 as the working concentration in following study. The survival cells were collected after BGC-823 cells were treated by doxorubicin for 48 hours，then cultured for 2-4 weeks and observed the morphologic changes. The inhibitory effects of doxorubicin on BGC-823 and BGC-823 DSCs for 48-hour was evaluated by MTT assay; the capacity of proliferation and metastasis between two group cells were analyzed by clone-forming assay, transwell assay and cell scratch assay. The proportions of SP cells in BGC-823 and BGC-823 DSCs were evaluated by immunofluorescence. The expression of ABCG2 and proportion of ABCG2+ cells in the two group cells were assessed by flow cytometry. Results: Doxorubicin inhibited BGC-823 cell proliferation in a dose-dependent manner. The IC50 and IC70 value at 48-hour on BGC-823 was 0.28μg/ml, 0.45μg/ml, respectively. While IC50 and IC70 value at 48-hour on BGC-823 DSCs was 0.51μg/ml, 0.73μg/ml, respectively. The BGC-823 DSCs showed low clone-forming capacity than the BGC-823 cells. In comparison to BGC-823 cells, BGC-823 DSCs showed high capacity of migration which was detected by Transwell assay and cell scratch assay. The SP cell and ABCG2+cell proportions were higher in BGC-823 DSCs than BGC-823 cells. And the expression of ABCG2 was increased in BGC-823 DSCs. Conclusion: BGC-823 DSCs showed high capacity of drug resistance and migration than BGC-823 cells; the SP cells and ABCG2+cells were enriched in the BGC-823 DSCs accompany high expression of ABCG2.