采用Miseq测序与碳平衡的生物滴滤床降解甲苯分析

bench scale biotrickling filter reactor was steadily operated to treat 1.64 mg/L of toluene. Nearly 84% toluene removal and the removal capacity of 166.41 g/（m3oh）was achieved when the EBRT was 30s and the sprinkle density was 0.812 m3/(m2 h). In this study, the biodegradation mechanism of a biotrickling filter treating toluene was studied by carbon equilibrium and Miseq-16S rRNA sequencing analysis. Under the stable operating conditions, 22.27% of carbon resource was assimilated into biomass, and 59.52% of carbon was degraded into CO2. Only 2.46% of carbon was dissolved in the nutrient solution. Miseq-16S rRNA sequencing manifested that the introduction of toluene decreased the biodiversity of bacterial community compared with the raw petrochemical sludge. Pseudomonas, Comamonadaceae, and Rhodococcus were the three predominant genera in the bioreactor fed with toluene. From the perspective of phyla, Proteobacteria, Bacteroidetes and Acidobacteria were the three dominant phyla in the bioreactor and among them, Proteobacteria and Bacteroidetes demonstrated opposite response to the introduction of toluene.