氟虫腈对斑马鱼miR-155及部分预测靶基因表达影响

Recent years,many research on mechanism of pesticides has been growing through biochemistry and molecule biology methods. Report shows that biomolecules such as DNA and RNA can be effected by pesticides.MicroRNAs(miRNAs) are small RNAs with the length of about 22nt,which play an important role in regulation of gene expression.Data shows that expression of zebrafish miR-155 could be changed by fipronil,while its effect on posible target genes is still unknown.Three potential target genes of zebrafish miR-155（cyb561d2、LOC570587 and zgc158824 ）was chosen from databases, Expression of these genes relative to miR-155 was tested by real-time PCR. Result shows,when expression of zebrafish miR-155 in zebrafish eyes was downregulated in the fipronil group,gene cyb561d2 was upregulated obviously-about six times, LOC570587 upregulated about two times, zgc158824 showed hardly any change.While in their brains, cyb561d2 was upregulated about four times, LOC570587 was downregulated about 0.8 times, zgc158824 also had little change.