重吸附法回收利用纤维素酶的工艺研究

he effect of Tween 80 addition, enzyme loading and substrate concentration on the re-adsorption of cellulases, which hydrolyzed the corncob after the pretreatment by soaking in aqueous ammonia, was studied in this paper. As the addition of Tween 80 and enzyme loading increased, the cellulase recovery first increased and then decreased gradually. The cellulase recovery increased gradually with the increasing concentration of the substrate. The optimum conditions were drawn as follows: Tween 80 addition of 0.25 g/g cellulose, enzyme loading of 40 FPU/g glucan, substrate concentration of 3.6 wt%. Under this condition, 83.0 % cellulase was recovered. This research provided a good solution to reduce the cellulase cost.