三角褐指藻对磷限制响应的分子机制的蛋白组学分析

Phosphorus (P), as an important macronutrient, is essential for the survival of marine phytoplankton. To further understand the phytoplankton response to the phosphorus limitation in the ocean at the cellular and molecular levels, proteomics profiling was carried out in the marine diatom Phaeodactylum tricornutum. Total 44 significantly changed nonredundant proteins were identified, among which 9 proteins were upregulated and the other 35 were downregulated. Interestingly, proteins associated with inorganic phosphate uptake were downregulated, while the protein involved in organic phosphorus uptake was upregulated, such as the alkaline phosphatase. Generally, except that a few metabolic responses were elevated, such as stress-shock proteins, protein degradation, lipid accumulation, and photorespiration, etc; many more metabolic activities tended to be downregulated, including energy metabolism,photosynthesis, amino acid and nucleic acid metabolisms, etc. Taking all into consideration, this work directly showed how P. tricornutum coordinated the multiple metabolic pathways adapted to phosphorus stress at the protein level, hence improving our knowledge of the role of P in the marine biogeochemical cycles and the phytoplankton response to P limitation in the ocean. Also, it provides some insight into the succession of phytoplankton community, providing a scientific basis for elucidating the mechanism of algal blooms.