健脾益气摄血方对ITP小鼠血清β-EP、VIP、5-HT、NE、CCK影响研究

Objectives: To investigate the effect of prescription JIANPIYIQISHEXUE on β-EP, VIP, 5-HT, NE and CCK in serum from ITP mice model. Methods: The ITP mice immune model was established. Prednisone and prescription JIANPIYIQISHEXUE (granules) were given, and the changes of β-EP, VIP, 5-HT, NE and CCK in serum were detected using ELISA method. Results: ①β-EP: β-EP in serum was up-regulated in ITP group (p<0.05); compared with ITP group, there was no significant change in prednisone group (p>0.05), but was recovered in JIANPIYIQISHEXUE group (p<0.05). ②VIP: VIP in serum was down-regulated in ITP group (p<0.05); but was recovered both in prednisone group and JIANPIYIQISHEXUE group (p<0.05). ③5-HT: 5-HT from serum was reduced in ITP group (p<0.05); and were augmented in prednisone group (p<0.01) and JIANPIYIQISHEXUE group (p<0.05). ④NE: The content of NE increased in ITP group and also after administration (p<0.05). ⑤CCK: The content of CCK in serum had no significant change after modeling and administration. Conclusions: The neurotransmitter and vasoactive substances including β-EP, VIP, 5-HT, NE were involved in the pathogenesis of ITP mice model, prescription JIANPIYIQISHEXUE effectively regulate the content of β-EP, VIP, 5-HT and NE. CCK did not participate in the process of ITP model.