六味地黄汤对快速衰小鼠认知功能及Wnt3a、GSK-3β表达的影响

Objective:To study the effects of Liuwei dihuang decoction on recognition in aging mice of D - galactose (D - gal) and the express of Wnt3a、GSK-3β.Methods: The rapid aging animal models was established by d-galactose subcutaneously injected drugs as 500 mg/(kg o d), the animals were randomly divided into normal group, model group, the high , Middle and low dose group of Liuwei dihuang decoction, VitE group (positive control group), to give different treatment. Lavage for volume (0.1 ml / 10 g), eight weeks, to observe the mice body quality change, line Morris water maze test evaluation of cognitive function in mice,The expression of Wnt3a and GSK-3βwas detected by immunohistochemical method and RT-PCR method. Results: Compared with the other groups, model group's quality of body weight (P < 0.05), less down longer latency and more (P < 0.05); Liuwei dihuang decoction group compared with normal group, there was no significant difference betweenthe high quality of body weight, the incubation period, and the collapse of frequency (P > 0.05);Low dose group of Liuwei dihuang decoction compared with VitE group of body quality and the ability of learning and memory also have no statistical significance (P > 0.05);The results were highly consistent with the detection of RT-qPCR, In model group, the expression of Wnt3a less, GSK-3βactivity，compared with normal group and treatment group difference was statistically significant (P < 0.01), High expression of Wnt3a in high dose group, GSK-3βactivity was lower than low dose group and VitE group, and it was highly significant (P<0.01)., Conclusion:liuwei dihuang decoction can improve the D - galactose rapid aging mice learning memory disorders; Increased expression of Wnt3a in aging mice, activation of the Wnt signaling pathway, down GSK-3beta expression may be the Liuwei Dihuang Decoction delaying senescence of Jiannao Yizhi mechanism.?????