Members of Venezuelan Equine Encephalitis Complex entry into host cells by clathrin-mediated endocytosis in a pH-dependent manner
Members of Venezuelan Equine Encephalitis Complex entry into host cells by clathrin-mediated endocytosis in a pH-dependent manner
Abstract Pixuna virus (PIXV) and Río Negro virus (RNV) are mosquito-borne alphaviruses belonging to the Venezuelan Equine Encephalitis (VEE) complex, which includes pathogenic epizootic and enzootic subtypes responsible for life-threatening diseases in equines. Considering that the first steps in viral infection are crucial for the efficient production of new progeny, the aim of this study was to elucidate the early events of the replication cycle of these two viruses. To this end, we used chemical inhibitors and the expression of dominant-negative constructs to study the dependence of clathrin and endosomal pH on PIXV and RNV internalization mechanisms. We demonstrated that both viruses are internalized primarily via clathrin-mediated endocytosis, where the low pH in endosomes is crucial for viral replication. Contributing knowledge regarding the entry route of VEE complex members is important to understand the pathogenesis of these viruses and also to develop new antiviral strategies.
Gil Pedro Ignacio、Quinteros Paloma Olmos、Gomez Emiliano、Piris Franco Martin、Paglini Maria Gabriela、Kunda Patricia、Ghietto Lucia Maria、Contigiani Marta
Instituto de Virolog¨aa ?°Dr. JM Vanella?±, Facultad de Ciencias M¨|dicas, Universidad Nacional de C¨?rdobaInstituto de Virolog¨aa ?°Dr. JM Vanella?±, Facultad de Ciencias M¨|dicas, Universidad Nacional de C¨?rdobaInstituto de Virolog¨aa ?°Dr. JM Vanella?±, Facultad de Ciencias M¨|dicas, Universidad Nacional de C¨?rdobaInstituto de Virolog¨aa ?°Dr. JM Vanella?±, Facultad de Ciencias M¨|dicas, Universidad Nacional de C¨?rdobaInstituto de Virolog¨aa ?°Dr. JM Vanella?±, Facultad de Ciencias M¨|dicas, Universidad Nacional de C¨?rdoba||Instituto de Investigaci¨?n M¨|dica Mercedes y Mart¨an Ferreyra, INIMEC-CONICET-Universidad Nacional de C¨?rdobaInstituto de Virolog¨aa ?°Dr. JM Vanella?±, Facultad de Ciencias M¨|dicas, Universidad Nacional de C¨?rdoba||Centro de Investigaci¨?n en Medicina Traslacional ?°Severo Amuch¨￠stegui?± (CIMETSA), Instituto Universitario Ciencias Biom¨|dicas C¨?rdoba (IUCBC)Instituto de Virolog¨aa ?°Dr. JM Vanella?±, Facultad de Ciencias M¨|dicas, Universidad Nacional de C¨?rdobaInstituto de Virolog¨aa ?°Dr. JM Vanella?±, Facultad de Ciencias M¨|dicas, Universidad Nacional de C¨?rdoba
微生物学分子生物学生物科学研究方法、生物科学研究技术
Pixuna virusRio Negro virusAlphavirusentryendosomes
Gil Pedro Ignacio,Quinteros Paloma Olmos,Gomez Emiliano,Piris Franco Martin,Paglini Maria Gabriela,Kunda Patricia,Ghietto Lucia Maria,Contigiani Marta.Members of Venezuelan Equine Encephalitis Complex entry into host cells by clathrin-mediated endocytosis in a pH-dependent manner[EB/OL].(2025-03-28)[2025-04-28].https://www.biorxiv.org/content/10.1101/2022.03.11.483939.点此复制
评论