菌剂施用对高温胁迫下芒萁光合与抗逆生理特性的影响

In order to investigate study the influence of microbial agent Bacillus natto on theability of Dicranopteris pedata to tolerate high temperature stress, this study determined thephysiological indexes of photosynthesis and resistance to high temperature of annual D. pedataunder different temperatures and fertilizers treatments. The results showed that: (1) bothtemperature and fertilizer could significantly affect the photosynthesis and high temperatureresistance physiology of D. pedata (P0.05). (2) The net photosynthetic rate, transpiration rate,stomatal conductance, and chlorophyll content of D. pedata leaves were significantly reduced (P0.05) when the temperature was increased to 45. The inhibition of photosynthetic physiologyof D. pedata by high-temperature stress was an non-stomatal limiting. To defend the hightemperature stress, the superoxide dismutase, peroxidase activity, proline, malondialdehydecontent, and relative conductivity of D. pedata were increased significantly (P 0.05). (3)Compared with the control group and the organic fertilizer group, the fertilizer with microbialagent audition significantly increased the net photosynthetic rate, transpiration rate, stomatalconductance, chlorophyll content, and the activities of peroxide dismutase and catalase in leavesof D. pedata, and decreased the intercellular CO2 concentration, malondialdehyde content andthe relative electrical conductivity of D. pedata leaves (P < 0.05). (4) The evaluation methodcombining principal component analysis and membership function method was used to evaluatethe high temperature resistance of D. pedata. It was found that the D. pedata scored higher inresistance to high temperature under fertilizer-added origin manure application. Overall, D. pedata has a certain degree of resistance to high temperature, and can selectively induce stress resistancephysiology to adapt the high temperatures environment according to different stress environments.The Bacillus natto can alleviate the photosynthesis inhibition of D. pedata by high temperaturestress, induce the increase of antioxidant enzyme activities to alleviate cell damage, reduce thepressure of osmoregulation, and effectively improved the resistance of D. pedata induced by hightemperature stress. This study provide a reference for the ecological restoration and soil and waterconservation in the southern red soil erosion area and similar areas.