SRAP、TRAP、SSR、ISSR分子标记在水稻籼粳分型中的系统学研究

Rice (Oryza sativa L.) is an important food crop, which is a constituent of more than 50% of the different varieties of food preparations in China. In order to exploit the utilization of heterosis between rice subspecies, 47 varieties of rice were classified into indica and japonica types firstly, using sequence-related amplified polymorphism (SRAP) and target region amplification polymorphism (TRAP) markers and then simple sequence repeat (SSR) and inter simple sequence repeat (ISSR) markers. Based on differences in the level of polymorphisms detected by the 4 markers-SRAP > ISSR > SSR >TRAP. The average genetic similarity of SRAP is 0.70, TRAP is 0.74, SSR 0.75, ISSR 0.85. which showed highly significant correlation between different markers.The subspecies could be differentiated by cluster analysis while some plants were different in 4 Phylogenetic trees. The result indicated that SRAP and TRAP have potential application in rice research and SRAP has more differentiated ability between varieties that had a near relationship.The javanica rice Lemont and P002 were belong to indica clade. Some wild rice varieties characterized as indica-like or japonica-like in cluster analysis with different markers; however, the cluster analysis of the data sets of the 4 markers combined , all the wild rice varieties were characterized as japonica-like. This indicated that the indica japonica differentiation of Chinese common wild rice varieties were not completed though the japonica-like tendency was happened. )