人参皂甙Rb1通过增加AQP4表达治疗SCII的研究

Objectives To investigate the effect of ginsenosTreatment SCII with ginsenoside Rb1 by increasing AQP4 expressionide Rb1 on spinal cord ischemia-reperfusion injury of rats and explore its mechanism. Methods Ninety-six Sprague-Dawley rats were randomly divided into four groups with twenty-four rats in each group. The groups were: blank group; sham operation; SCII group; and ginsenoside Rb1- treated group. In ginsenoside Rb1- treated group, intraperitoneal injection with ginsenoside Rb1 10mg/kgod for 7days. At 1, 3, 5, or 7 days after reperfusion started, six rats of every group were then killed, and the following studies were performed: the scores of spinal cord injury was determined by BBB system, the organizational structure were analyzed by HE staining, and the apoptosis rate was measured by Tunel. The AQP4 expression was detected with immunofluorescence and Real-time PCR. Results The BBB score of ginsenoside Rb1- treated group was higher than that of SCII group. Ginsenoside Rb1 had protected effects on the morphous of neurone, and compared with the SCII group, the congestion of tissue space and cellular swelling were signifficant reduced. Tunel also found ginsenoside Rb1 treatment can significantly reduced apoptosis compared with SCII group. The dynamic change of AQP4 in SCII was significantly decreased from 1d to 7d, especially in 1d. With ginsenoside Rb1 treatment, AQP4 expression could notably been up-regulated. These results indicate that ginsenoside Rb1 could partly block the AQP4 reduction causing by SCII. Conclusion Ginsenoside Rb1 had treatment effects on SCII. The possible mechanism may be involved with up-regulation AQP4 expression, reduction nerve adema and apoptosis.