马铃薯蛋白水解物中抗氧化肽的主成分分析预测及分离纯化验证

o predict the antioxidative peptides present in potato protein hydrolysate, amino acid composition of potato protein hydrolysate fractions with different molecular weight and polarity was used as the variate, and potential peptide sequences were predicted using principal component analysis. Ultrafiltration and chromtograpy seperation was combined with tandem mass spectrum to purify, identify and verify the antioxidative peptides in potato protein hydrolysate based on the ABTS+o scavenging assay. The results showed that the amino acid residues including Tyr, Phe and His were predicted to be the composition in antioxidative peptides. Those three amino acids were present in the sequence of the identified antioxidative peptides Phe-Tyr, Tyr-Tyr, Tyr-Phe-Glu and Pro-Pro-His-Tyr-Phe which were released from patatin. It indicated that combination multiple variate analysis with amino acid composition of protein hydrolysate was able to predict the antioxidative peptide sequence.