GIRK1基因在宫颈癌中的表达及临床意义

Objectives To study the expression of GIRK1 mRNA in cervical cancer. Methods Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of GIRK1 mRNA in 60 patients with cervical cancer,10 patients with cervical cancer before and after radiotherapy and chemotherapy, and 10 patients with normal cervical tissue. Results the ratio of gray level of GIRK1 mRNA was 0.0252 in normal cervical tissue,1.5472 in cervical cancer,there were significant differences between them (P<0.01).The ratio of gray level of GIRK1 mRNA was 1.8627 in the poor differentiation cancer, higher than that of the middle and high differentiation cancer 0.9855 and 0.8564(P<0.05). the ratio of gray level of GIRK1 mRNA was 0.9896 in stages Ⅰ～Ⅱ,1.9742 in stages Ⅲ～Ⅳ,there were significant differences between them (P<0.01).GIRK1 mRNA was 1.3710 before radiotherapy and chemotherapy, but 0.2939 after radiotherapy and chemotherapy, they have significant differences(P<0.01). Conclusion GIRK1 mRNA participates in the proliferation of cervical cancer cell, and plays an important role in tumorgenesis of cervical cancer. GIRK1 mRNA have a positive correlation with clinical stages and pathological grades of cervical cancer. The expression of GIRK1 mRNA in cervical cancer was decreased after radiotherapy and chemotherapy.