蔬菜中硫代葡萄糖苷酶解条件的研究

In order to improve the efficiency of glucosinolates degradated to sulforaphane, the effects of several factors on enzymolysis of glucosinolate were studied, and the optimum enzymatic degradation conditions were determinated in this paper. The optimum conditions for glucosinolate direct enzymolysis by endogenous myrosinase were: liquid-solid ratio, 3mL/g; time, 8 hours; temperature, 25℃; pH, 4.0; and ascorbic acid, 0.02 mg/g. The optimum conditions for glucosinolate indirect enzymolysis by exogenous myrosinase were: enzyme-glucosinolate ratio, 1000mL/g; time, 3 hours; temperature, 35℃; pH, 5.0; and ascorbic acid, 0.02 mg/g. Comparing the optimum conditions of two enzymolysis methods of glucosinolate, the advantages and disadvantages were obtained. This study is useful for glucosinolate enzymolysis and the preparative-scale isolation of sulforaphane.